Function Based Metagenomics Approach for Obtaining Lipase from Acid Mine Drainage Site

Havva Esra Tütüncü, Nevin Gul Karaguler, Merve Oztug, Nurgul Celik Balci, Melek Tuter


Metagenomics is the culture independent study of microbial populations present in any environment. The number of newly discovered enzymes increases rapidly with the advances in metagenomics. The studies of metagenomics are either accomplished by a sequence based or function based approach. In sequence based approach, either samples are directly sequenced and they are associated with some functions, or new genes are found by using the knowledge about already identified homologous sequences. In function based approach, a metagenomic library is constructed and each clone is screened in terms of function or sequence.

In this work, environmental samples from an acid mine drainage site (Balıkesir, Turkey) were collected. Microbial population was enriched and metagenomic library was constructed using fosmid vectors. About 12000 clones were screened in terms of lipolytic activity and two of them showed positive result. To further analyze the samples, partial sequencing, preliminary functional characterization and subcloning strategies were applied. In this report, it is aimed to serve the recent developments in metagenomics and exemplify functional metagenomic strategies, with an emphasis on the acidophilic samples

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