Optimization of Cultivation and Supercritical Fluid Lipid Extraction Conditions of Schizochytrium sp. S31

Nurcan Vardar, Yiğit Ayhan, Emrah Yelboğa, Banu Bahar , Melek Tüter, Nevin Gül Karagüler

Abstract


Schizochytrium sp. is widely studied microalgae to obtain high content of polyunsaturated fatty acids (PUFAs) especially docosahexaenoic acid (22:6n-3) by cultivation optimization. In this study, cultivation and supercritical fluid lipid extraction conditions were optimized to maximize biomass and total lipid content of the Schizochytrium sp. S31, a known docosahexaenoic acid (DHA) producing marine algae by using response surface methodology. The traditional extraction methods have low extraction yields and use large amounts of toxic solvents. Supercritical fluid extraction (SFE) technique provide higher selectivity, shorter extraction times and do not use toxic organic solvents.

Based on contour plots and canonical analysis, a maximum biomass production of 26.86g/L was obtained with 2.29 g/L monosodium glutamate (MSG), pH 5.8 cultivation conditions. Maximum lipid production of 35% was obtained with 0.49 g/LMSG at 17.6 °C temperature conditions.  According to SFE results, pressure and temperature has significant effect (p <0.05) on total lipid yield and DHA concentration. It shows that, at constant temperature lipid yield and DHA concentration increases with higher pressure whereas decreases with higher temperature. After optimization of pressure, temperature and time variables of SFE, 30.2% lipid yield was obtained. In compare with traditional extraction, SFE has the advantages of to get solvent free value-added extracts.

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DOI: http://dx.doi.org/10.1000/ijses.v2i4.83

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